HTS Biological Assays Unit
The High-Throughput Screening (HTS) and Biological Assays Unit at the BCDD specializes in in vitro assay development and automation for the HTS campaigns.
The facility offers state-of-the-art instrumenation for High Content Imaging, cell culturing, compound handling and plate reading.
Our collaborative approach ensures that all our technologies, instrumentation, resources and expertise are effectively utilized to address researcher's discovery needs.
Infrastructure
The HTS room
Two integrated Freedom EVO 150 and EVO 200 Tecan robots equipped with: 2 Robotic Manipulation arms, liquid handling 8 channels unit, liquid handling 96 channels unit, Carousel for 220 microplates and deepwell plates, Centrifuge, TECAN Hydrospeed 96/384 plate washer, Multidrop reagent dispenser, Tecan infinite M1000 Pro-Multi mode plate reader, Chill/heat plate, plate shaker, plate incubator MIO2 system, Pin Tools for TECAN MCA96, Lyconic 44 plates CO2 incubator, TECAN HP300 digital dispenser, In Cell 2200 image Analyzer (GE Healthcare), Quant Studio 12K Flex (Applied Biosystems), 3 PC computers and specific software licenses for TECAN, IN Cell 2200 and Quant studio 12K Flex for applications programing, running and data analysis.
The TC room
3 MS12 Laminar HEPA-flow biological cabinets, 4 HERA-Cell VIOS 160l CO2 Incubators (Thermo Scientific), TC microscope (Zeiss), Cell counter, Water bath, bench 4C° refrigerator and -20C° freezer.
The cryo room
Freezers -20C° and -80C° - Revco (Thermo Scientific), Double door 4C° refrigerator for TC reagent storage, Liquid nitrogen storage for 6000 samples and backup nitrogen tank, ice-maker, Laboratory freeze dryer - ALPHA 1-4 LD plus.