Biological Services

Biological Services
Biological Services at the BCDD

Assay Development

  • Technology transfer to HTS
  • Assay adaptation to 96, 384 or 1536 well plate format
  • Establishing an automation protocol for HTS screening
  • Assay validation and data analysis
  • Detailed report containing full SOP (Standard Operating Procedure) will be delivered upon project completion

 

Assay Development

  • HTS Screening of 1-100K from commercial libraries (~ 200,000 available compounds) or a dedicated library provided by the client
  • HTS hits validation: concentration dependent assay, developing a counter screen 

 

Systems

  • An integrated robotic system consisting TEACAN freedom EVO 150 and EVO 200 dedicated for enzymatic and cell-based assays
  • A TEACAN freedom EVO 150 robotic system dedicated for yeast and bacterial assays
  • BIACORE T200
  • Thermo-Fisher QuantStudio 12K Flex Real-Time PCR System

 

Cell-based Assays

  • Dual/split luciferase
  • Intercellular protein-protein interactions
  • Proliferation  
  • Activation
  • Apoptosis
  • Autophagy
  • Cell Adhesion and attachment
  • Cell migration: Chemotaxis, Haptotaxis, Transmigration, Wound healing
  • Macrophage activation assays (Phagocytosis, Oxidative burst assay)
  • Cytokine assays
  • Transient and stable transfections
  • Induction of cell differentiation
  • Single step Q-PCR
  • High content imaging
    • Target expression levels
    • Target localization
    • Organelle localization
    • Organelle distribution
  • Cell and organelle size and shape
  • Cell Viability, cytotoxicity
  • Cell toxicity and viability assays- MTT,XTT, CellTiter Glo® Luminescent
  • CYP450 induction/inhibition assays -DDI assessment in the early developing stage of new compounds
  • hERG channels assay- for early identification of hERG liability in drug discovery

 

Protein-protein interactions Assays

  • TR-FRET, BRET, BiFC, AlphaScreen
  • SPR analysis- BIACORE

 

Immuno-based assays

  • Elisa/immunoblots
  •  Functional assays
  • Antibody screening

 

Enzymatic-based Assays

  • Mono-and multi-substrate reactions
  • Kinetic analysis - determination of Km, Vmax

 

Microbial and Yeast-based assays

  • A TEACAN freedom EVO 150 robotic system dedicated for yeast and bacterial assays.
  • This system is capable of incubating large number of parallel cultures and monitor their growth as well as measure fluorescence and luminescence-based signals.

 

Cell culture services

  • A fully equipped BSL2 tissue culture room is available for the preparation of biological materials for diverse drug screening assays.
    • The culture room is equipped with 3 MS12 Laminar HEPA-flow biological cabinets, 4 HERA-Cell VIOS 160l CO2 Incubators (Thermo Scientific), TC microscope (Zeiss), Cell counter, Water bath, and more.
  • Cell culture of multiple cell types modeling disease,  including NCI-60 Human Tumor Cell Lines;
    • Utilizes different human tumor cell lines, representing leukemia, melanoma, cancers of the lung, colon, brain, ovary, breast, prostate, and kidney cancers.
    • Screen can be conducted against control strains from the appropriate sources (i.e. colon, CNS and others).
    • XTT kit is being used for quantification of cellular proliferation, viability, and cytotoxicity.
       
Tel Aviv University makes every effort to respect copyright. If you own copyright to the content contained
here and / or the use of such content is in your opinion infringing, Contact us as soon as possible >>