Biological Services

Biological Services
Biological Services at the BCDD

Assay Development

  • Technology transfer to HTS
  • Assay adaptation to 96, 384 or 1536 well plate format
  • Establishing an automation protocol for HTS screening
  • Assay validation and data analysis
  • Detailed report containing full SOP (Standard Operating Procedure) will be delivered upon project completion

 

Assay Development

  • HTS Screening of 1-100K from commercial libraries (~ 200,000 available compounds) or a dedicated library provided by the client
  • HTS hits validation: concentration dependent assay, developing a counter screen 

 

Systems

  • An integrated robotic system consisting TEACAN freedom EVO 150 and EVO 200 dedicated for enzymatic and cell-based assays
  • A TEACAN freedom EVO 150 robotic system dedicated for yeast and bacterial assays
  • BIACORE T200
  • Thermo-Fisher QuantStudio 12K Flex Real-Time PCR System

 

Cell-based Assays

  • Dual/split luciferase
  • Intercellular protein-protein interactions
  • Proliferation  
  • Activation
  • Apoptosis
  • Autophagy
  • Cell Adhesion and attachment
  • Cell migration: Chemotaxis, Haptotaxis, Transmigration, Wound healing
  • Macrophage activation assays (Phagocytosis, Oxidative burst assay)
  • Cytokine assays
  • Transient and stable transfections
  • Induction of cell differentiation
  • Single step Q-PCR
  • High content imaging
    • Target expression levels
    • Target localization
    • Organelle localization
    • Organelle distribution
  • Cell and organelle size and shape
  • Cell Viability, cytotoxicity
  • Cell toxicity and viability assays- MTT,XTT, CellTiter Glo® Luminescent
  • CYP450 induction/inhibition assays -DDI assessment in the early developing stage of new compounds
  • hERG channels assay- for early identification of hERG liability in drug discovery

 

Protein-protein interactions Assays

  • TR-FRET, BRET, BiFC, AlphaScreen
  • SPR analysis- BIACORE

 

Immuno-based assays

  • Elisa/immunoblots
  •  Functional assays
  • Antibody screening

 

Enzymatic-based Assays

  • Mono-and multi-substrate reactions
  • Kinetic analysis - determination of Km, Vmax

 

Microbial and Yeast-based assays

  • A TEACAN freedom EVO 150 robotic system dedicated for yeast and bacterial assays.
  • This system is capable of incubating large number of parallel cultures and monitor their growth as well as measure fluorescence and luminescence-based signals.

 

Cell culture services

  • A fully equipped BSL2 tissue culture room is available for the preparation of biological materials for diverse drug screening assays.
    • The culture room is equipped with 3 MS12 Laminar HEPA-flow biological cabinets, 4 HERA-Cell VIOS 160l CO2 Incubators (Thermo Scientific), TC microscope (Zeiss), Cell counter, Water bath, and more.
  • Cell culture of multiple cell types modeling disease,  including NCI-60 Human Tumor Cell Lines;
    • Utilizes different human tumor cell lines, representing leukemia, melanoma, cancers of the lung, colon, brain, ovary, breast, prostate, and kidney cancers.
    • Screen can be conducted against control strains from the appropriate sources (i.e. colon, CNS and others).
    • XTT kit is being used for quantification of cellular proliferation, viability, and cytotoxicity.
       
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