Biological Services

Assay Development

• Technology transfer to HTS
• Assay adaptation to 96, 384 or 1536 well plate format
• Establishing an automation protocol for HTS screening
• Assay validation and data analysis
• Detailed report containing full SOP (Standard Operating Procedure) will be delivered upon project completion

Assay Development

• HTS Screening of 1-100K from commercial libraries (~ 200,000 available compounds) or a dedicated library provided by the client
• HTS hits validation: concentration dependent assay, developing a counter screen 

Systems

• An integrated robotic system consisting TEACAN freedom EVO 150 and EVO 200 dedicated for enzymatic and cell-based assays
• A TEACAN freedom EVO 150 robotic system dedicated for yeast and bacterial assays
• BIACORE T200
• Thermo-Fisher QuantStudio 12K Flex Real-Time PCR System

Cell-based Assays

• Dual/split luciferase
• Intercellular protein-protein interactions
• Proliferation  
• Activation
• Apoptosis
• Autophagy
• Cell Adhesion and attachment
• Cell migration: Chemotaxis, Haptotaxis, Transmigration, Wound healing
• Macrophage activation assays (Phagocytosis, Oxidative burst assay)
• Cytokine assays
• Transient and stable transfections
• Induction of cell differentiation
• Single step Q-PCR
• High content imaging
  • Target expression levels
  • Target localization
  • Organelle localization
  • Organelle distribution
• Cell and organelle size and shape
• Cell Viability, cytotoxicity
• Cell toxicity and viability assays- MTT,XTT, CellTiter Glo® Luminescent
• CYP450 induction/inhibition assays -DDI assessment in the early developing stage of new compounds
• hERG channels assay- for early identification of hERG liability in drug discovery

Protein-protein interactions Assays

• TR-FRET, BRET, BiFC, AlphaScreen
• SPR analysis- BIACORE

Immuno-based assays

• Elisa/immunoblots
•  Functional assays
• Antibody screening

Enzymatic-based Assays

• Mono-and multi-substrate reactions
• Kinetic analysis - determination of Km, Vmax

Microbial and Yeast-based assays

• A TEACAN freedom EVO 150 robotic system dedicated for yeast and bacterial assays.
• This system is capable of incubating large number of parallel cultures and monitor their growth as well as measure fluorescence and luminescence-based signals.

Cell culture services

• A fully equipped BSL2 tissue culture room is available for the preparation of biological materials for diverse drug screening assays.
  • The culture room is equipped with 3 MS12 Laminar HEPA-flow biological cabinets, 4 HERA-Cell VIOS 160l CO2 Incubators (Thermo Scientific), TC microscope (Zeiss), Cell counter, Water bath, and more.
• Cell culture of multiple cell types modeling disease,  including NCI-60 Human Tumor Cell Lines;
  • Utilizes different human tumor cell lines, representing leukemia, melanoma, cancers of the lung, colon, brain, ovary, breast, prostate, and kidney cancers.
  • Screen can be conducted against control strains from the appropriate sources (i.e. colon, CNS and others).
  • XTT kit is being used for quantification of cellular proliferation, viability, and cytotoxicity.