Biological Services

Assay Development

• Technology transfer to HTS
• Assay adaptation to 96, 384, or 1536 well plate format
• Establishing an automation protocol for HTS screening
• Assay validation and data analysis
• Detailed report containing full SOP (Standard Operating Procedure) will be delivered upon project completion

Cell-Based Assays

• Dual/split luciferase
• Intercellular protein-protein interactions
• Proliferation  
• Activation
• Apoptosis
• Autophagy
• Cell Adhesion and attachment
• Cell migration: Chemotaxis, Haptotaxis, Transmigration, Wound healing
• Macrophage activation assays (Phagocytosis, Oxidative burst assay)
• Cytokine assays
• Transient and stable transfections
• Induction of cell differentiation
• Single-step Q-PCR
• High content imaging
  • Target expression levels
  • Target localization
  • Organelle localization
  • Organelle distribution
• Cell and organelle size and shape
• Cell Viability, cytotoxicity
• Cell toxicity and viability assays- MTT, XTT, CellTiter Glo® Luminescent
• CYP450 induction/inhibition assays -DDI assessment in the early developing stage of new compounds
• hERG channels assay- for early identification of hERG liability in drug discovery

Protein-Protein Interactions Assays

• TR-FRET, BRET, BiFC, AlphaScreen
• SPR analysis- BIACORE

Immuno-Based Assays

• Elisa/immunoblots
•  Functional assays
• Antibody screening

Enzymatic-Based Assays

• Mono-and multi-substrate reactions
• Kinetic analysis - determination of Km, Vmax

Microbial and Yeast-Based Assays

• A TEACAN freedom EVO 150 robotic system dedicated to yeast and bacterial assays
• This system is capable of incubating large number of parallel cultures and monitor their growth as well as measure fluorescence and luminescence-based signals